Prof. Dr. Enikő Forró

Research topic:

Development of new enzyme-catalysed kinetic resolution methods

Supervisor:
Prof. Dr. Enikő Forró

Description:
Development of new enzyme-catalysed kinetic resolution methods (a maximum yield of 50% enantiopure product) for the preparation of enantiomeric compounds, such as amino acids, amino esters, lactams and amino alcohols, through the enantioselective ring cleavage of the corresponding racemic activated or inactivated lactams, the enantioselective hydrolysis of amino esters or the asymmetric acylation of the OH group of amino alcohols. Synthesis of the racemic starting substances. Development of adequate analytical methods with which to follow the enzymatic reactions [using a gas- (GC) or liquid-chromatograph (HPLC) fitted with chiral columns]. Investigation of the biocatalytic processes, and identification of the most suitable biocatalysts and best reaction conditions, in batch and continuous-flow systems. Gram-scale resolutions (under optimized conditions).

 

Research topic:
Development of new enzyme-catalysed kinetic and dinamic kinetic resolution

Supervisor:
Prof. Dr. Enikő Forró

Description:
Development of new enzyme-catalysed kinetic and dinamic kinetic resolution (a maximum yield of 100% enantiopure product) strategies for the preparation of enantiomeric b-amino acids, important from both pharmacological and chemical aspects. Investigation of the enzymatic hydrolysis of racemic b-amino esters in water and in organic solvents. Development and optimization of the enzymatic reactions in a solvent-free system. Study of potentially of substrate engineering. Gram-scale resolutions (with the most efficient biocatalyst and optimized reaction conditions).